
(At least you had better hope it is.) Aboutġ0% of the nitrate in the soil is produced by lightning strikes, cosmic radiationĪnd meteor trails.
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To know where the nitrates come from and how to control them. This is called the "blue-baby disease." We need Sensitive to this problem and will turn a bluish color just as if they wereĪsphyxiating. To die because they cannot get the oxygen they need. If the methemoglobin level rises above 3% the cells begin The normal level of methemoglobin in the humanīody is about 2%. The human body changes nitrate to nitrite. If people ingest too much nitrate, they may become sick. Plants can use the nitrogen in the form of nitrite (NO 2 -) This is why most fertilizers have high concentrations of nitrogen. Plants need nitrogen in forms that they can get through It is necessary for the growth and metabolism These are to:īACKGROUND Nitrogen, Nitrites, Nitrates and Bacteria

Then, the experiment must progress for several days, with This activity may take several weeks to complete. Grow denitrifying bacteria out of the soil.

Monitor the rate of change of nitrate in the soil as a result of bacterial action.Nitrate (to show that this concentration of contaminant is not directly detectable Water standard to be exceeded by preparing a solution containing 10 mg/L of Demonstrate that only a very small amount of contaminant can cause the drinking.To accomplish the above objectives this module provides four student-oriented laboratory experiences. Grow bacteria out of the soil that can metabolize nitrateĭevelop the pre-requisite lab skills that are used in bioremediation Four Experiments Monitor and correlate the activity of bacteria with nitrate levels in the soil This module would be suitable for a class project at the upperĭetermine nitrate levels in soils obtained from a variety To grow and isolate denitrifying bacteria from the soil. It can be used to teach a variety of laboratory techniques needed by beginning science students. The focus of the module concerns Nitrate Levels In The Soil. In this learning module, students will perform activities that demonstrate some of the principles ofīioremediation of soil. LABORATORY ACTIVITY 4: OBSERVING THE DENITRIFIERS IN ACTIONĪPPENDIX B: PERIODIC TABLE OF THE ELEMENTSĪPPENDIX C: DETERMINING DRY MASS AND MOISTURE CONTENTĪPPENDIX D: CHART FOR MONITORING NITRATE LEVELS OVER TIMEĪPPENDIX G: GROWING DENITRIFYING BACTERIA FROM THE SOIL. LABORATORY ACTIVITY 3: OBSERVING THE EFFECTS FO BACTERIAL LABORATORY ACTIVITY 2: TESTING SOIL FOR NITRATE

LABORATORY ACTIVITY 1: A DEMONSTRATION: HOW MUCH IS TOO MUCH?
